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Product Details
Synonyms
S1 protein NTD, Spike protein S1 NTD, BetaCoV S1-NTD
Source
Biotinylated SARS-CoV-2 Spike NTD, His,Avitag (SPD-C82H3) is expressed from human 293 cells (HEK293). It contains AA Ser 13 - Leu 303 (Accession # QHD43416.1 (T19R, G142D, EF156-157del, R158G)). The mutations T19R, G142D, EF156-157del, R158G were identified in the SARS-CoV-2 Delta variant (Pango lineage: B.1.617.2).
Predicted N-terminus: Ser 13
Request for sequenceMolecular Characterization
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 36.3 kDa. The protein migrates as 45-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the SABA assay / HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated SARS-CoV-2 Spike NTD, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Biotinylated SARS-CoV-2 Spike NTD, His,Avitag (Cat. No. SPD-C82H3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb (Cat. No. SPD-M121) with a linear range of 0.1-2 ng/mL (QC tested).
Protocol
Immobilized Anti-SARS-CoV-2 Spike NTD Antibody, Chimeric mAb (Cat. No. SPD-M121) at 1 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Spike NTD, His,Avitag (Cat. No. SPD-C82H3) with a linear range of 0.8-13 ng/mL (Routinely tested).
Protocol
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FAQ
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Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
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