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Your Position: Home > Receptor Tyrosine Kinases > FGF R2 (IIIb) > FGB-H5256

Human FGF R2 (IIIb) protein, Fc Tag (MALS verified)

  • Synonym
    FGF R2 (IIIb),FGFR2B,FGFR2
  • Source
    Human FGF R2 (IIIb), Fc Tag (FGB-H5256) is expressed from human 293 cells (HEK293). It contains AA Pro 154 - Leu 358 (Accession # P21802-3).
    Predicted N-terminus: Pro 154
  • Molecular Characterization
    Online(Pro 154 - Leu 358) P21802-3

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 49.4 kDa. The protein migrates as 65-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) SDS-PAGE gel

Human FGF R2 (IIIb), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human FGF R2 (IIIb), Fc Tag (Cat. No. ) MALS images

The purity of Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) is more than 90% and the molecular weight of this protein is around 119-145 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human FGF R2 (IIIb), Fc TagHuman FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) ELISA bioactivity

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 5 μg/mL (100 μL/well) can bind Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) with a linear range of 1-16 ng/mL (QC tested).

Human FGF R2 (IIIb), Fc TagHuman FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) ELISA bioactivity

Immobilized Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) with a linear range of 2-31 ng/mL (Routinely tested).

  • Background
    Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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