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Your Position: Home > Protein > FGF-7 > FG7-H82E7

Biotinylated Human FGF-7 / HBGF-7 / KGF Protein, His,Avitag™ (MALS verified)

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  • Synonym
    FGF-7,Fibroblast growth factor 7,HBGF-7,Keratinocyte growth factor,KGF
  • Source
    Biotinylated Human FGF-7, His,Avitag (FG7-H82E7) is expressed from human 293 cells (HEK293). It contains AA Cys 32 - Thr 194 (Accession # P21781-1).
    Predicted N-terminus: Cys 32
  • Molecular Characterization
    Online(Cys 32 - Thr 194) P21781-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 22.6 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human FGF-7, His,Avitag (Cat. No. FG7-H82E7) SDS-PAGE gel

Biotinylated Human FGF-7, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human FGF-7, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human FGF-7, His,Avitag (Cat. No. FG7-H82E7) is more than 90% and the molecular weight of this protein is around 31-47 kDa verified by SEC-MALS.

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human FGF-7, His,Avitag (Cat. No. FG7-H82E7) on SA Biosensor, can bind Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) with an affinity constant of 37 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human FGF-7, His,Avitag (Cat. No. FG7-H82E7) on SA Biosensor, can bind Human FGF R1, His Tag (Cat. No. FG1-H5223) with an affinity constant of 243 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Fibroblast growth factor (FGF) 7 (is also known as Keratinocyte growth factor (KGF)), a member of FGF family, is initially found to be secreted from mesenchymal cells to repair epithelial tissues. As a well-characterized paracrine growth factor for tissue growth and regeneration, fibroblast growth factor 7 (FGF7) is involved in a number of physiological and pathological processes, including lung disease and cancer. The stromal-derived FGFs, such as FGF7 and FGF10, control epithelial cell resident FGFR2IIIb activities, promote net tissue homeostasis, and restraint tumor cells from progression to malignancy.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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