This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 22.6 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human FGF-7, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human FGF-7, His,Avitag (Cat. No. FG7-H82E7) is more than 90% and the molecular weight of this protein is around 31-47 kDa verified by SEC-MALS.
Loaded Biotinylated Human FGF-7, His,Avitag (Cat. No. FG7-H82E7) on SA Biosensor, can bind Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) with an affinity constant of 37 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Loaded Biotinylated Human FGF-7, His,Avitag (Cat. No. FG7-H82E7) on SA Biosensor, can bind Human FGF R1, His Tag (Cat. No. FG1-H5223) with an affinity constant of 243 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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