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Product Details
Synonyms
FOLR2, BETA-HFR, FBP, PL-1, FR-BETA, FR-P3, FBP
Source
Biotinylated Human FOLR2, His,Avitag (FO2-H82E8) is expressed from human 293 cells (HEK293). It contains AA Thr 17 - His 228 (Accession # P14207-1).
Predicted N-terminus: Thr 17
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 28.2 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human FOLR2, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
Bioactivity-ELISA
Immobilized Folic acid-BSA conjugate at 5 μg/mL (100 μL/well) can bind Biotinylated Human FOLR2, His,Avitag (Cat. No. FO2-H82E8) with a linear range of 0.2-1 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Immobilized Folic acid-BSA on CM5 Chip can bind Biotinylated Human FOLR2, His,Avitag (Cat. No. FO2-H82E8) with an affinity constant of 4.61 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Folate receptor beta
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
2 Details
- Highest Development Stage：
Phase 3 Clinical
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