Human Elongin B&Elongin C&VHL Heterotrimer Protein, Strep II Tag&Strep II Tag&His Tag, produced by co-expression of Elongin B and Elongin C and VHL, has a calculated MW of 14.5 kDa (Elongin B) and 13.8 kDa (Elongin C) and 25.8 kDa (VHL). Subunit Elongin B is fused with a Strep II tag at the N-terminus and Elongin C is fused with a Strep II tag at the N-terminus and VHL is fused with a polyhistidine tag at the N-terminus. The protein migrates as 17 kDa (Elongin B) and 14 kDa (Elongin C) and 30 kDa (VHL) under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM HEPES, 200 mM NaCl, 20% Glycerol, 1 mM TCEP, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
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This product is stable after storage at:
Human Elongin B&Elongin C&VHL Heterotrimer Protein, Strep II Tag&Strep II Tag&His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human Elongin B&Elongin C&VHL Heterotrimer Protein, Strep II Tag&Strep II Tag&His Tag (Cat. No. ELL-H5595) is more than 85% and the molecular weight of this protein is around 51-63 kDa verified by SEC-MALS.
Human Elongin B&Elongin C&VHL Heterotrimer Protein, Strep II Tag&Strep II Tag&His Tag (Cat. No. ELL-H5595) immobilized on CM5 Chip can bind MZ1 with an affinity constant of 275 μM as determined in a SPR assay (Biacore 8K) (QC tested).
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