This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.8 kDa. The protein migrates as 31-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 24 months in lyophilized state;
-70°C for 12 months under sterile conditions after reconstitution.
Human PD-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human PD-1, His Tag(Cat. No. PD1-H5221) is more than 85% and the molecular weight of this protein is around 28-42 kDa verified by SEC-MALS.
Opdivo (Nivolumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human PD-1, His Tag (Cat. No. PD1-H5221) with an affinity constant of 4.94 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Loaded Human PD-1, His Tag (Cat. No. PD1-H5221) on HIS1K Biosensor, can bind Human PD-L1, Fc Tag (HPLC verified) (Cat. No. PD1-H5258) with an affinity constant of 38.9 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Loaded Human PD-1, His Tag (Cat. No. PD1-H5221) on HIS1K Biosensor, can bind Human PD-L2, Fc Tag (HPLC verified) (Cat. No. PD2-H5251) with an affinity constant of 16.3 nM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Loaded Opdivo (Nivolumab) on ProteinA Biosensor, can bind Human PD-1, His Tag (Cat. No. PD1-H5221) with an affinity constant of 5.09 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human PD-L1, Mouse IgG1 Fc Tag, low endotoxin (HPLC-verified) (Cat. No. PD1-H52A3) on AMC Biosensor, can bind Human Human PD-1, His Tag (Cat. No. PD1-H5221) with an affinity constant of 2.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Curnock, Adam P et al.
Journal: JCI Insight 2021
Application: Binding Assay
Authors: X Qian, et al
Journal: US20190330348A1 2019
Authors: Wang J, et al
Journal: Mabs 2019
Authors: G Galler, et al
Journal: US20190144542A1 2019
Authors: C Rothe, et al
Journal: US20190010231A1 2019
Authors: Yam Alice, et al.
Journal: US20180142022A1 2018
Authors: Wang Aijun, et al.
Journal: US20170355770A1 2017
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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