Human Integrin alpha D beta 2 (ITGAD&ITGB2) Heterodimer Protein, produced by co-expression of ITGAD and ITGB2, has a calculated MW of 124.9 kDa (ITGAD) and 80.2 kDa (ITGB2). Subunit ITGAD is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as 150 kDa (ITGAD) and 90-100 kDa (ITGB2) under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human ITGAD&ITGB2 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human ITGAD&ITGB2 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT2-H53W6) at 1 μg/mL (100 μL/well) can bind Human Integrin beta 2/CD18 Antibody with a linear range of 0.3-5 ng/mL (QC tested).
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