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Your Position: Home > Cytokines > Osteopontin > OPN-H82E8

Biotinylated Human Osteopontin (OPN) Protein, His,Avitag™

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    SPP1,BNSP,OPN,Uropontin,Nephropontin,Osteopontin,BSP-1,ETA-1,BSPI
  • Source
    Biotinylated Human Osteopontin, His,Avitag (OPN-H82E8) is expressed from human 293 cells (HEK293). It contains AA Ile 17 - Asn 300 (Accession # P10451-5).
    Predicted N-terminus: Ile 17
  • Molecular Characterization
    Online(Ile 17 - Asn 300) P10451-5

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 35.7 kDa. The protein migrates as 16 kDa, 23 kDa, 28 kDa and 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and proteolytic processing of several intracellular enzymes.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >80% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Osteopontin, His,Avitag (Cat. No. OPN-H82E8) SDS-PAGE gel

Biotinylated Human Osteopontin, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 80%.

Bioactivity-ELISA
Biotinylated Human Osteopontin, His,AvitagBiotinylated Human Osteopontin, His,Avitag (Cat. No. OPN-H82E8) ELISA bioactivity

Immobilized Human ITGAV&ITGB3 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT3-H52E3) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Osteopontin, His,Avitag (Cat. No. OPN-H82E8) with a linear range of 0.078-5 μg/mL (Routinely tested).

  • Background
    Osteopontin (OPN) is also known as Secreted phosphoprotein 1 (SPP1), Bone sialoprotein 1, Nephropontin, Urinary stone protein, Uropontin, BNSP, which belongs to the osteopontin family. OPN / SPP1 is a highly negatively charged, extracellular matrix protein that lacks an extensive secondary structure. Full length OPN (OPN-FL) can be modified by thrombin cleavage, which exposes a cryptic sequence, SVVYGLR on the cleaved form of the protein known as OPN-R. Osteopontin / SPP-1 is biosynthesized by a variety of tissue types. OPN is the ligand for integrin alpha-V/beta-3. OPN / SPP1 binds tightly to hydroxyapatite and appears to form an integral part of the mineralized matrix. OPN / SPP1 probably important to cell-matrix interaction. OPN / SPP1 acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 2 business days

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  • Latest Research Phase:Discontinued

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