Biotinylated Human Osteopontin (OPN) Protein, His,Avitag™

Synonyms

SPP1, BNSP, OPN, Uropontin, Nephropontin, Osteopontin, BSP-1, ETA-1, BSPI

Source

Biotinylated Human Osteopontin, His,Avitag (OPN-H82E8) is expressed from human 293 cells (HEK293). It contains AA Ile 17 - Asn 300 (Accession # P10451-5).

Predicted N-terminus: Ile 17

Molecular Characterization

Other Tags and Version Biotin & Other Labeled Version

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 35.7 kDa. The protein migrates as 16 kDa, 23 kDa, 28 kDa and 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation and proteolytic processing of several intracellular enzymes.

Labeling

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Protein Ratio

Passed as determined by the HABA assay / binding ELISA.

Purity

>80% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;
2. -70°C for 3 months under sterile conditions after reconstitution.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process