Human IL-18 R beta Protein, Fc Tag

AIDD‑Ready
Related Products: Cell line CHEK-ATF233
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ILB-H5256-100ug
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ILB-H5256-1mg (500ug X 2)
$1,720.00
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ILB-H5256
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Synonyms

IL-18 R beta, IL-1 R7, CD218b, CDw218b, IL-1 R7, IL-18 R beta, IL-18 receptor accessory protein, IL-18 receptor beta, IL18R beta, IL-18RAcP

Source

Human IL-18 R beta Protein, Fc Tag (ILB-H5256) is expressed from human 293 cells (HEK293). It contains AA Phe 20 - Arg 356 (Accession # O95256-1).

Predicted N-terminus: Phe 20

Molecular Characterization

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 64.9 kDa. The protein migrates as 70-100 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>95% as determined by SDS-PAGE.

Formulation

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

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Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.

Background

IL-18 R beta (IL-1R7), also known as IL18RAP (Interleukin 18 Receptor Accessory Protein), is a member of the IL-1 family. Within the IL18 receptor complex, does not mediate IL18-binding, but involved in IL18-dependent signal transduction, leading to NF-kappa-B and JNK activation