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Your Position: Home > Cytokines > IL-27 > IL7-M82E4

Biotinylated Mouse IL-27 Protein, His,Avitag™

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    p28,IL30,IL-27,IL-27A,IL27p28,Interleukin-27,EBI3
  • Source
    Biotinylated Mouse IL-27 Protein, His,Avitag (IL7-M82E4) is expressed from human 293 cells (HEK293). It contains AA Tyr 19 - Pro 228 (IL-27B) & Phe 29 - Ser 234 (IL-27A) (Accession # O35228-1(IL-27B) & Q8K3I6-1(IL-27A)).
    Predicted N-terminus: Tyr 19
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 52.2 kDa. The protein migrates as 55 kDa (IL-27B) & 60 kDa (IL-27A) under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM MOPS, 150 mM NaCl, 0.05% CHAPS, 1mM EDTA, pH7.0 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse IL-27 Protein, His,Avitag (Cat. No. IL7-M82E4) SDS-PAGE gel

Biotinylated Mouse IL-27 Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Mouse IL-27 Protein, His,AvitagBiotinylated Mouse IL-27 Protein, His,Avitag (Cat. No. IL7-M82E4) ELISA bioactivity

Immobilized Human IL-27 Ra, Fc Tag (Cat. No. ILA-H5254) at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IL-27 Protein, His,Avitag (Cat. No. IL7-M82E4) with a linear range of 0.2-4 ng/mL (QC tested).

  • Background
    Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits, Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells. IL-27 plays an important function in regulating the activity of B and T lymphocytes. The effects of IL-27 are eliciting by its interaction with a specific cell surface receptor complex composed of two proteins known as IL27R and gp130. IL-27 is a cytokine with pro- and anti-inflammatory properties, that can regulate T helper cell development, suppress T-cell proliferation, stimulate cytotoxic T cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T cells. Another important role of IL-27 is its antitumor activity and antiangiogenic activity.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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