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Biotinylated Human ICAM-1 / CD54 Protein, His,Avitag™ (MALS verified)

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Cat. No. / Size
Price
Qty
IC1-H82E8-25ug
$360.00
IC1-H82E8-200ug
$1565.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Synonyms

    ICAM1, BB2, CD54, P3.58

  • Source

    Biotinylated Human ICAM-1, His,Avitag (IC1-H82E8) is expressed from human 293 cells (HEK293). It contains AA Gln 28 - Glu 480 (Accession # P05362-1).

    Predicted N-terminus: Gln 28

    Request for sequence
  • Molecular Characterization

    ICAM-1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 53.1 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    ICAM-1 SDS-PAGE

    Biotinylated Human ICAM-1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    ICAM-1 SEC-MALS

    The purity of Biotinylated Human ICAM-1, His,Avitag (Cat. No. IC1-H82E8) is more than 85% and the molecular weight of this protein is around 65-80 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     ICAM-1 ELISA

    Immobilized Human ITGAL&ITGB2 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT2-H53W3) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ICAM-1, His,Avitag (Cat. No. IC1-H82E8) with a linear range of 0.001-0.625 μg/mL (QC tested).

    Protocol

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Background

Inter-Cellular Adhesion Molecule 1 (ICAM-1) is also known as Cluster of Differentiation 54 (CD54), is a member of the immunoglobulin superfamily, and is a cell surface glycoprotein which is typically expressed in low concentrations on endothelial cells and cells of the immune system. The protein encoded by this gene is a type of intercellular adhesion molecule continuously present in low concentrations in the membranes of leukocytes and endothelial cells. Upon cytokine stimulation, the concentrations greatly increase. ICAM-1 can be induced by interleukin-1 (IL-1) and tumor necrosis factor alpha (TNFα) and is expressed by the vascular endothelium, macrophages, and lymphocytes. ICAM-1 is a ligand for LFA-1 (integrin), a receptor found on leukocytes. When activated, leukocytes bind to endothelial cells via ICAM-1/LFA-1 and then transmigrate into tissues. ICAM-1 has been implicated in subarachnoid hemorrhage (SAH). Levels of ICAM-1 are shown to be significantly elevated in patients with SAH over control subjects in many studies. ICAM-1 expressed by respiratory epithelial cells is also the binding site for rhinovirus, the causative agent of most common colds.

Recent Advances

 
Drug Development Progress
  • English Name:

    Intercellular adhesion molecule-1

  • Category:

  • Approved Drugs:

    2 Details

  • Drugs in Clinical Trials:

    4 Details

  • Highest Development Stage:

    Approved

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