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Your Position: Home > CAR-T > FOLR1 > FO1-HP2H9

PE-Labeled Human FOLR1 Protein, His Tag (Site-specific conjugation)

  • Synonym
    FOLR-1,FBP,FOLR,FRα
  • Source
    PE-Labeled Human FOLR1, His Tag (FO1-HP2H9) is produced via site-specific conjugation of PE to Human FOLR1, His Tag under optimal conditions with a proprietary technology. Human FOLR1, His Tag is expressed from human 293 cells (HEK293). It contains AA Arg 25 - Met 233 (Accession # P15328-1).
    Predicted N-terminus: Arg 25
  • Molecular Characterization
    Online(Arg 25 - Met 233) P15328-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 28.3 kDa.

  • Application
    Evaluation of anti-FOLR1 CAR expression by flow cytometry. Please note that this product is NOT compatible to streptavidin detection system.
  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

5e5 of anti-FOLR1 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human FOLR1, His Tag (Cat. No. FO1-HP2H9) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).

  • Background
    Folate Receptor 1 (FOLR1) is also known as Folate receptor alpha, Folate Binding Protein (FBP), FOLR, and is a member of the folate receptor (FOLR) family. Members of this gene family have a high affinity for folic acid and for several reduced folic acid derivatives, and mediate delivery of 5-methyltetrahydrofolate to the interior of cells. Mature FOLR1 is an N-glycosylated protein that is anchored to the cell surface by a GPI linkage. FOLR1 is predominantly expressed on epithelial cells and is dramatically upregulated on many carcinomas. FOLR1 is internalized to the endosomal system where it dissociates from its ligand before recycling to the cell surface. A soluble form of FOLR1 can be proteolytically shed from the cell surface into the serum and breast milk. Defects in FOLR1 are the cause of neurodegeneration due to cerebral folate transport deficiency (NCFTD). NCFTD is an autosomal recessive disorder resulting from brain-specific folate deficiency early in life.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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