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Your Position: Home > Protein > Glycoprotein (NiV, HeV) > GLN-N52H3

Nipah virus Glycoprotein G, His Tag (MALS verified)

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  • Synonym
    Glycoprotein
  • Source
    Nipah virus Glycoprotein G, His Tag(GLN-N52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 71 - Thr 602 (Accession # Q9IH62-1 ).
    Predicted N-terminus: His
  • Molecular Characterization
    Glycoprotein (NiV, HeV) Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 61.2 kDa. The protein migrates as 70-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Glycoprotein (NiV, HeV) SDS-PAGE

Nipah virus Glycoprotein G, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Glycoprotein (NiV, HeV) MALS images

The purity of Nipah virus Glycoprotein G, His Tag (Cat. No. GLN-N52H3) is more than 90% and the molecular weight of this protein is around 73-90 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Glycoprotein (NiV, HeV) ELISA

Immobilized Nipah virus Glycoprotein G, His Tag (Cat. No. GLN-N52H3) at 5 μg/mL (100 μL/well) can bind Human Ephrin-B2 Protein, Fc tag (Cat. No. EPN-H5259) with a linear range of 0.1-2 ng/mL (QC tested).

 Glycoprotein (NiV, HeV) ELISA

Immobilized Nipah virus Glycoprotein G, His Tag (Cat. No. GLN-N52H3) at 1 μg/mL (100 μL/well) can bind Anti-Nipah/Hendra Glycoprotein G Antibody, Human IgG1 with a linear range of 0.03-1 ng/mL (Routinely tested).

Bioactivity-SPR
 Glycoprotein (NiV, HeV) SPR

Anti-Nipah/Hendra Glycoprotein G Antibody, Human IgG1 captured on Protein A Chip can bind Nipah virus Glycoprotein G, His Tag (Cat. No. GLN-N52H3) with an affinity constant of 0.188 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Hendra virus (HeV) and Nipah virus (NiV) are henipaviruses discovered in the mid-to late 1990s that possess a broad host tropism and are known to cause severe and often fatal disease in both humans and animals. HeV and NiV infect host cells through the coordinated efforts of two envelope glycoproteins. The G glycoprotein attaches to cell receptors, triggering the fusion (F) glycoprotein to execute membrane fusion. G is a type II homotetrameric transmembrane protein responsible for binding to ephrinB2 or ephrinB3 (ephrinB2/B3) receptors. F is a homotrimeric type I transmembrane protein that is synthesized as a premature F0 precursor and cleaved by cathepsin L during endocytic recycling to yield the mature, disulfide-linked, F1 and F2 subunits. Upon binding to ephrinB2/B3, NiV G undergoes conformational changes leading to F triggering and insertion of the F hydrophobic fusion peptide into the target membrane. Subsequent refolding into the more stable post-fusion F conformation drives merger of the viral and host membranes to form a pore for genome delivery to the cell cytoplasm.
  • Clinical and Translational Updates

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