Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 21.8 kDa. The protein migrates as 30-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag (Cat. No. 41B-C82E9) is more than 90% and the molecular weight of this protein is around 27-41kDa verified by SEC-MALS.
Immobilized Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag (Cat. No. 41B-C82E9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Cynomolgus 4-1BB Ligand, Fc Tag (Cat. No. 41L-C5254) with a linear range of 0.3-2 ng/mL (QC tested).
Immobilized Human 4-1BB Ligand, Fc Tag (Cat. No. 41L-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus / Rhesus macaque 4-1BB, Avitag,His Tag (Cat. No. 41B-C82E9) with a linear range of 0.6-20 ng/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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