Login Register
icon_bulk_orderBulk order Acrobiosystems for English
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > TME Relevant Pathway > Integrin alpha V beta 8 > IT8-C52W9

Cynomolgus Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein, His Tag&Tag Free

  • Synonym
    Integrin alpha V beta 8,ITGAV&ITGB8
  • Source
    Cynomolgus ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free (IT8-C52W9) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Pro 993 (ITGAV) & Glu 43 - Ser 681 (ITGB8) (Accession # A0A2K5WCD3-1(ITGAV) & G7P0S0-1(ITGB8)).
    Predicted N-terminus: Phe 31 (ITGAV) & Glu 43 (ITGB8)
  • Molecular Characterization
    Online(Phe 31 - Pro 993 (ITGAV) & Glu 43 - Ser 681 (ITGB8)) A0A2K5WCD3-1 (ITGAV) & G7P0S0-1 (ITGB8)

    Cynomolgus Integrin alpha V beta 8 (ITGAV&ITGB6) Heterodimer Protein, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 113.1 kDa (ITGAV) and 76.0 kDa (ITGB8). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The predicted N-terminus is Phe 31 (ITGAV) & Glu 43 (ITGB8). The non-reducing (NR) protein migrates as 135-150 kDa (ITGAV) & 75-85 kDa (ITGB8) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT8-C52W9) SDS-PAGE gel

Cynomolgus ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Cynomolgus ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag FreeCynomolgus ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT8-C52W9) ELISA bioactivity

Immobilized Cynomolgus ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT8-C52W9) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGF-Beta 1, His,Avitag (Cat. No. TG1-H82Qb) with a linear range of 0.5-16 ng/mL (QC tested).

  • Background
    Integrin alpha V beta 8 (ITGAV & ITGB8 or ITGAVB8) is expressed in yolk sac, placenta, brain perivascular astrocytes, Schwann cells, renal glomerular mesangial cells and pulmonary epithelial cells. Unlike other alpha V integrins, ITGAVB8 does not appear to assume different activation states, and the cytoplasmic tail does not connect to the cytoskeleton. It binds ligands containing an RGD motif, including vitronectin, fibrin and the latency associated peptide (LAP) of the latent TGF-beta complex. High affinity binding of alpha V beta 8 to LAP allows proteolytic cleavage by MT1-MMP, which releases active TGF-beta. This mechanism differs from that of alpha V beta 6, the other alpha V integrin which can activate TGF-beta from latency through non-proteolytic mechanisms. Downstream effects of TGF-beta activation include control of cell growth and associated vascularization.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)


ETD of in-stock products: 2 business days

Price(USD) : $410.00

Price(USD) : $2880.00

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America: +1 800-810-0816 (Toll Free)
Asia & Pacific: +86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message