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Your Position: Home > Cytokines > IL-2 > IL2-H5269

Human IL-2 Protein, Fc Tag (MALS verified)

  • Synonym
    IL2,TCGF,lymphokine,Interleukin 2
  • Source
    Human IL-2 Protein, Fc Tag (IL2-H5269) is expressed from human 293 cells (HEK293). It contains AA Ala 21 - Thr 153 (Accession # P60568-1).
    Predicted N-terminus: Ala 21
  • Molecular Characterization
    Online(Ala 21 - Thr 153) P60568-1

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 41.9 kDa. The protein migrates as 45-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IL-2 Protein, Fc Tag (Cat. No. IL2-H5269) SDS-PAGE gel

Human IL-2 Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human IL-2 Protein, Fc Tag (Cat. No. ) MALS images

The purity of Human IL-2 Protein, Fc Tag (Cat. No. IL2-H5269) is more than 90% and the molecular weight of this protein is around 80-98kDa verified by SEC-MALS.

Bioactivity-ELISA
Human IL-2 Protein, Fc TagHuman IL-2 Protein, Fc Tag (Cat. No. IL2-H5269) ELISA bioactivity

Immobilized Human IL-2 R alpha, His Tag (Cat. No. ILA-H52H9) at 5 μg/mL (100 μL/well) can bind Human IL-2 Protein, Fc Tag (Cat. No. IL2-H5269) with a linear range of 0.1-2 ng/mL (QC tested).

Human IL-2 Protein, Fc TagHuman IL-2 Protein, Fc Tag (Cat. No. IL2-H5269) ELISA bioactivity

Immobilized Human IL-2 Protein, Fc Tag (Cat. No. IL2-H5269) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2 R alpha, His,Avitag (Cat. No. ILA-H82E6) with a linear range of 0.3-20 ng/mL (Routinely tested).

Human IL-2 Protein, Fc TagHuman IL-2 Protein, Fc Tag (Cat. No. IL2-H5269) ELISA bioactivity

Immobilized Human IL-2 Protein, Fc Tag (Cat. No. IL2-H5269) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-2 R beta, His,Avitag (Cat. No. ILB-H82E3) with a linear range of 0.005-1.25 μg/mL (Routinely tested).

  • Background
    Interleukin-2 (IL-2) is an interleukin, a type of cytokine immune system signaling molecule, which is a leukocytotrophic hormone that is instrumental in the body's natural response to microbial infection and in discriminating between foreign (non-self) and self. IL-2 mediates its effects by binding to IL-2 receptors, which are expressed by lymphocytes, the cells that are responsible for immunity. Mature human IL-2 shares 56% and 66% aa sequence identity with mouse and rat IL-2, respectively. Human and mouse IL-2 exhibit crossspecies activity. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying preformed complexes. IL-2 is also necessary during T cell development in the thymus for the maturation of a unique subset of T cells that are termed regulatory T cells (T-regs). After exiting from the thymus, T-Regs function to prevent other T cells from recognizing and reacting against "self antigens", which could result in "autoimmunity". T-Regs do so by preventing the responding cells from producing IL-2. Thus, IL-2 is required to discriminate between self and non-self, another one of the unique characteristics of the immune system.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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