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Product Details
Synonyms
IL3, MCGF, MGC79398, MGC79399, MULTI-CSF, Interleukin-3
Source
Human IL-3 Protein, His Tag (IL3-H52H9) is expressed from human 293 cells (HEK293). It contains AA Ala 20 - Phe 152 (Accession # P08700-1).
Predicted N-terminus: Ala 20
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 17.0 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.3 EU per μg by the LAL method / rFC method.
Sterility
Negative
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human IL-3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Human IL-3 Protein, His Tag (Cat. No. IL3-H52H9) is more than 85% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human IL-3 R alpha, Fc,Avitag (Cat. No. ILA-H82F3) at 5 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-3 Protein, His Tag (Cat. No. IL3-H52H9) with a linear range of 0.01-0.313 μg/mL (QC tested).
Protocol
Bioactivity-BLI
Loaded Human IL-3 R alpha, Fc Tag (Cat. No. ILA-H5252) on Protein A Biosensor, can bind Human IL-3 Protein, His Tag (Cat. No. IL3-H52H9) with an affinity constant of 0.71 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Protocol
Loaded Biotinylated Human IL-3 R alpha, Fc,Avitag (Cat. No. ILA-H82F3) on SA Biosensor, can bind Human IL-3 Protein, His Tag (Cat. No. IL3-H52H9) with an affinity constant of 0.35 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
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