|RAS045-C01||Pre-coated with SARS-CoV-2 Spike S1 Microplate||1 plate|
|RAS045-C02||Anti-SARS-CoV-2 Antibody (Control, Mouse IgG)||100 μL|
|RAS045-C03||Positive Control||100 μL|
|RAS045-C04||Negative Control||100 μL|
|RAS045-C05||HRP-Goat anti-Mouse IgG||10 μg|
|RAS045-C06||10×Washing Buffer||50 mL|
|RAS045-C07||Dilution Buffer||50 mL|
|RAS045-C08||Substrate Solution||12 mL|
|RAS045-C09||Stop Solution||7 mL|
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 4 simple steps:
a) The samples and Control are diluted by Dilution Buffer.Add your sample to the plate.
b) Add diluted Secondary antibody HRP-Goat anti-Mouse IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calc μLated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Mouse IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike S1 at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Mouse IgG in 1:100 mouse serum. Detection was performed using HRP-Anti-mouse IgG antibody with sensitivity of 9.8 ng/mL (QC tested).
Price(USD) : $580.00
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