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Your Position: Home > All Other Proteins > CD3E & CD3G > CDG-H82W3

Biotinylated Human CD3 epsilon&CD3 gamma Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)

Based on the high binding affinity between biotin-streptavidin systems, our best seller SP-11 Streptavidin Coated Plates are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    CD3 epsilon & CD3 gamma,CD3E & CD3G
  • Source
    Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free (CDG-H82W3) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G) (Accession # NP_000724.1(CD3E) & AAI13831(CD3G)).
    Predicted N-terminus: Asp 23 (CD3E)
  • Molecular Characterization
    Online(Asp 23 - Asp 126 (CD3E) & Gln 23 - Ser 116 (CD3G)) NP_000724.1 (CD3E) & AAI13831 (CD3G)

    Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free is produced by co-expression of CD3E and CD3G, has a calculated MW of 18.6 kDa (CD3E) and 14.7 kDa (CD3G). Subunit CD3E is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag and subunit CD3G contains no tag at the C-terminus. The reducing (R) heterodimer protein migrates as 22 kDa and 24-30 kDa due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDG-H82W3) SDS-PAGE gel

Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free (Cat. No. ) MALS images

The purity of Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDG-H82W3) is more than 90% and the molecular weight of this protein is around 35-52kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag FreeBiotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDG-H82W3) ELISA bioactivity

Immobilized Mouse Anti-Human CD3 (SP34-2) at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDG-H82W3) with a linear range of 0.016-0.25 μg/mL (QC tested).

Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag FreeBiotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDG-H82W3) ELISA bioactivity

Immobilized UCHT1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human CD3E&CD3G Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDG-H82W3) with a linear range of 0.5-31 ng/mL (QC tested).

  • Background
    T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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