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Your Position: Home > Others > FOLR4 > FO4-H52H3

Human FOLR4 Protein, His Tag (MALS verified)

  • Synonym
    folate receptor 4 (delta) homolog (mouse),Folbp3,FOLR4,FR-delta,LOC390243,probable folate receptor delta
  • Source
    Human FOLR4, His Tag (FO4-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gly 20 - Ser 228 (Accession # A6ND01-1).
    Predicted N-terminus: Gly 20
  • Molecular Characterization
    Online(Gly 20 - Ser 228) A6ND01-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 25.8 kDa. The protein migrates as 30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 500 mM NaCl, pH7.5 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human FOLR4, His Tag (Cat. No. FO4-H52H3) SDS-PAGE gel

Human FOLR4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human FOLR4, His Tag (Cat. No. ) MALS images

The purity of Human FOLR4, His Tag (Cat. No. FO4-H52H3) is more than 85% and the molecular weight of this protein is around 24-36kDa verified by SEC-MALS.

  • Background
    FOLR4, also know as Sperm-egg fusion protein Juno(IZUMO1R) is receptor for IZUMO1 present at the cell surface of oocytes (oolemma), which is essential for species-specific gamete recognition and fertilization. The IZUMO1:IZUMO1R/JUNO interaction is a necessary adhesion event between sperm and egg that is required for fertilization but is not sufficient for cell fusion. The ligand-receptor interaction probably does not act as a membrane 'fusogen'. Does not bind folate.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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