This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 110.8 kDa. The protein migrates as 125-145 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mouse Klotho beta, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Loaded Human FGF19, Fc Tag (Cat. No. FG9-H5253) on Protein A Biosensor, can bind Mouse Klotho beta, His Tag (Cat. No. KLB-M52H7) with an affinity constant of 78.6 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human FGF R4, Fc Tag (Cat. No. FG4-H5253) on Protein A Biosensor, can bind Mouse Klotho beta, His Tag (Cat. No. KLB-M52H7) with an affinity constant of 54.0 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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