This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 73.3 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human LRP-5, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human SOST, His Tag (Cat. No. HST-H5245) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) with a linear range of 0.078-1.25 μg/mL (Routinely tested).
Loaded Biotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) on SA Biosensor, can bind Human SOST, His Tag (Cat. No. HST-H5245) with an affinity constant of 7.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Biotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) on SA Biosensor, can bind Human Dkk-1, His Tag (Cat. No. DK1-H5221) with an affinity constant of 93.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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