Login Register
icon_bulk_orderBulk order Acrobiosystems for English
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > TME Relevant Pathway > LRP-5 > LR5-H82E6

Biotinylated Human LRP-5 Protein, His,Avitag™

Recommended Hot Products: SP-11 Streptavidin Coated Plates, Clear, 96-Well  STN-N5116 Recombinant Streptavidin  SMB-B01 Magnetic Beads™ Streptavidin 
Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    LRP5,LRP-5,LRP-7,LRP7,LR3
  • Source
    Biotinylated Human LRP-5, His,Avitag (LR5-H82E6) is expressed from human 293 cells (HEK293). It contains AA Glu 644 - Gln 1263 (Accession # O75197-1 ).
    Predicted N-terminus: Glu 644
  • Molecular Characterization
    Online(Glu 644 - Gln 1263) O75197-1 

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 73.3 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) SDS-PAGE gel

Biotinylated Human LRP-5, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Biotinylated Human LRP-5, His,AvitagBiotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) ELISA bioactivity

Immobilized Human SOST, His Tag (Cat. No. HST-H5245) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) with a linear range of 0.078-1.25 μg/mL (Routinely tested).

Biotinylated Human LRP-5, His,AvitagBiotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) ELISA bioactivity

Immobilized Human Dkk-1, His Tag (Cat. No. DK1-H5221) at 5 μg/mL (100 μL/well) can bind Biotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) with a linear range of 0.078-1.25 μg/mL (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) on SA Biosensor, can bind Human SOST, His Tag (Cat. No. HST-H5245) with an affinity constant of 7.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Biotinylated Human LRP-5, His,Avitag (Cat. No. LR5-H82E6) on SA Biosensor, can bind Human Dkk-1, His Tag (Cat. No. DK1-H5221) with an affinity constant of 93.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    Low-density lipoprotein receptor-related protein 5(LRP-5) is also known as BMND1, EVR1, EVR4, HBM, LR3, LRP7, OPPG, OPTA1, VBCH2, LDL receptor related protein 5 and PCLD4. LRP5 is a transmembrane low-density lipoprotein receptor that shares a similar structure with LRP6. LRP5 acts as a co-receptor with LRP6 and the Frizzled protein family members for transducing signals by Wnt proteins through the canonical Wnt pathway. This protein plays a key role in skeletal homeostasis. Mutations in LRP5 can lead to considerable changes in bone mass. A loss-of-function mutation causes osteoporosis-pseudoglioma (decrease in bone mass), while a gain-of-function mutation causes drastic increases in bone mass.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)


ETD of in-stock products: 2 business days

Price(USD) : $350.00

Price(USD) : $1380.00

Price(USD) : $30.00

Price(USD) : $130.00

Price(USD) : $210.00

Price(USD) : $210.00

Price(USD) : $350.00

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America: +1 800-810-0816 (Toll Free)
Asia & Pacific: +86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message