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Your Position: Home > variant-specific antibody > Spike RBD > SPD-M334

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AM334) (Beta & Gamma Specific) (MALS verified)

  • Source
    Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Beta & Gamma Specific) (SPD-M334) is a chimeric monoclonal antibody recombinantly expressed from HEK293 cells, which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody was obtained from a mouse immunized with recombinant SARS-CoV-2 Spike RBD protein. The antibody is specific against the Beta (B.1.351) and Gamma (P.1) variant of SARS-CoV-2, and has no binding with the spike RBD of the wild type virus, the Alpha (B.1.1.7) variant, the Delta (B.1.617.2) variant and the Omicron (B.1.1.529, BA.2) variant.
  • Isotype
    Mouse IgG1 | Mouse Kappa
  • Specificity
    This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. No cross-reactivity is detected with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • Shipping
    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.
SDS-PAGE
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Beta & Gamma Specific) (Cat. No. SPD-M334) SDS-PAGE gel

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Beta & Gamma Specific) on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Beta & Gamma Specific) (Cat. No. ) MALS images

The purity of Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Beta & Gamma Specific) (Cat. No. SPD-M334) is more than 90% and the molecular weight of this protein is around 130-155 kDa verified by SEC-MALS.

Bioactivity-ELISA
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Beta & Gamma Specific)Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Beta & Gamma Specific) (Cat. No. SPD-M334) ELISA bioactivity

Immobilized SARS-CoV-2 spike protein (Beta, Cat. No. SPN-C52Hk and Gamma, Cat. No. SPN-C52Hg) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Beta & Gamma Specific) (Cat. No. SPD-M334) with a linear range of 0.1-8 ng/mL. The antibody does not bind spike protein of WT (Cat. No. SPN-C52H7), Alpha (Cat. No. SPN-C52H6) and Delta (Cat. No. SPN-C52He) (QC tested).

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Beta & Gamma Specific)Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Beta & Gamma Specific) (Cat. No. SPD-M334) ELISA bioactivity

Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Beta & Gamma Specific) (Cat. No. SPD-M334) can bind SARS-CoV-2 spike protein (Beta, Cat. No. SPN-C52Hk and Gamma, Cat. No. SPN-C52Hg) with a linear range of 0.5-8 ng/mL. The antibody does not bind spike protein of WT (Cat. No. SPN-C52H7), Alpha (Cat. No. SPN-C52H6) and Delta (Cat. No. SPN-C52He) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Beta & Gamma Specific) (Cat. No. SPD-M334) loaded on AMC Biosensor can bind SARS-CoV-2 S protein, His Tag (Beta, Cat. No. SPN-C52Hk) with an affinity constant of 11.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Beta & Gamma Specific) (Cat. No. SPD-M334) loaded on AMC Biosensor can bind SARS-CoV-2 S protein, His Tag (Gamma, Cat. No. SPN-C52Hg) with an affinity constant of 13.1 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Beta & Gamma Specific) (Cat. No. SPD-M334) loaded on AMC Biosensor does not bind SARS-CoV-2 S protein, His Tag (WT, Cat. No. SPN-C52H7) as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Beta & Gamma Specific) (Cat. No. SPD-M334) loaded on AMC Biosensor does not bind SARS-CoV-2 S protein, His Tag (Alpha, Cat. No. SPN-C52H6) as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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