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Your Position: Home > Antigen kits > Spike RBD > RAS-A050

SARS-CoV-2 Spike RBD ELISA Kit (For Vaccine Development)

For research use only.
Materials Provided
IDComponentsSize
RAS050-C01Pre-coated Anti-SARS-CoV-2 Spike RBD Antibody Microplate1 plate
RAS050-C02SARS-CoV-2 Spike RBD10 μg
RAS050-C03HRP-Anti-SARS-CoV-2 Spike RBD Antibody20 μg
RAS050-C0410xWashing Buffer50 mL
RAS050-C05Dilution Buffer50 mL
RAS050-C06Substrate Solution12 mL
RAS050-C07Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of SARS-CoV-2 Spike Protein RBD is urgently needed to accelerate the development of COVID-19 vaccines.
  • Application
    This kit is developed for detecting SARS-CoV-2 Spike RBD in vaccine samples,witch is the low sensitivity version of Cat.No. RAS-A021.The kit can meet the needs of vaccine developers to establish antigen quantification methods for preclinical evaluation, vaccine production and quality control,and realize accurate quantification of vaccine antigen contents for COVID-19 vaccines of all technological platforms, multivalent vaccines, and VOC-specific boosters.
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.

  • Assay Principles
    This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike RBD. The kit consists of microplate pre-coated with Anti-SARS-CoV-2 Spike RBD Antibody, an SARS-CoV-2 Spike RBD as Control, an HPR-Anti-SARS-CoV-2 Spike RBD Antibody,and buffers.

    Your experiment will include 5 simple steps:

    a) All reagents were returned to room temperature(20℃-25℃) before use.

    b) Add your sample to the plate, take the SARS-CoV-2 Spike RBD as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    c) Add a diluted HRP-Anti-SARS-CoV-2 Spike RBD Antibody to the plate. The diluted by Dilution Buffer.

    d) Wash the plate and add TMB.

    e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

Typical Data
SARS-CoV-2 Spike RBD ELISA Kit (For Vaccine Development)SARS-CoV-2 Spike RBD ELISA Kit (For Vaccine Development) (Cat. No. RAS-A050) ELISA bioactivity

Detection of Monoclonal SARS-CoV-2 Spike RBD titer by sandwich-ELISA Assay.
Immobilized Anti-SARS-CoV-2 Spike RBD Antibody at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD. Detection was performed using HRP-Anti-SARS-CoV-2 Spike RBD Antibody with sensitivity of 2 ng/mL (QC tested).

SARS-CoV-2 Spike RBD ELISA Kit (For Vaccine Development)SARS-CoV-2 Spike RBD ELISA Kit (For Vaccine Development) (Cat. No. RAS-A050) ELISA bioactivity

SARS-CoV-2 Spike RBD ELISA Kit (For Vaccine Development) (Cat.No.RAS-A050) can potently to detect the all SARS-CoV-2 Variants of Concern (VOCs), including Omicron (Cat.No.SPD-C522e).

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Price(USD) : $580.00

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