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Your Position: Home > Kits > Spike protein > RAS-T060

Mouse Anti-SARS-CoV-2 (B.1.1.529) Antibody IgG Titer Serologic Assay Kit (Spike Trimer)

For research use only.

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IDComponentsSize
RAS060-C01Pre-coated SARS-CoV-2 Spike Trimer (B.1.1.529) Microplate1 plate
RAS060-C02SARS-CoV-2 Antibody Positive Control100 μL
RAS060-C03SARS-CoV-2 Antibody Negative Control100 μL
RAS060-C04HRP-Goat anti-Mouse IgG10 μg
RAS060-C0510xWashing Buffer50 mL
RAS060-C06Dilution Buffer50 mL
RAS060-C07Substrate Solution12 mL
RAS060-C08Stop Solution7 mL
  • Background
    As of Nov 29th, confirmed cases of a new variant of concern (VOC) B.1.1.529 have been reported in South Africa, Botswana and more than 12 other countries. Thanks to the rapid sequencing efforts of South African scientists, the whole world is now alerted about this potentially devastating lineage, which was assigned the name Omicron by WHO.Omicron is a highly divergent variant with a high number of mutations (26-35) in the spike. Identification of some familiar mutations that also appeared on other VOCs gives rises to concerns of its immune escape potential and higher transmissibility. To facilitate the mutant-related research, drug trials and vaccine development, a high-throughput assay to measure IgG antibodies against the mutants is in urgent need.
  • Application

    This kit is developed for titer measurement of Anti-SARS-CoV-2 (B.1.1.529) Antibody IgG (Spike Trimer) in Mouse serum.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage & Shipping
    1. Unopened kit should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    Shipping Statement

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in Mouse serum by SARS-CoV-2 Spike Trimer. The Kit consists of Pre-coated with SARS-CoV-2 Spike Trimer Microplate, an Positive Control, an Negative Control, an HRP-Anti-Goat anti mouse IgG secondary antibody.

    Your experiment will include 4 simple steps:

    a) The samples and Control are diluted by Dilution Buffer.Add your sample to the plate.

    b) Add diluted Secondary antibody HRP-Goat anti-Mouse IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

  • Clinical and Translational Updates

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ETA of in-stock products: 2 business days

Price(USD) : $650.00

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Approved

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