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Your Position: Home > TME Relevant Pathway > FGF-10 > FG0-H81Q7

Biotinylated Human FGF-10 / KGF 2 Protein, His,Avitag™ (SPR verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    FGF-10,Fibroblast growth factor 10,Keratinocyte growth factor 2
  • Source
    Biotinylated Human FGF-10, His,Avitag (FG0-H81Q7) is expressed from E.coli cells. It contains AA Gln 38 - Ser 208 (Accession # O15520-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    Online(Gln 38 - Ser 208) O15520-1

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 22.9 kDa. The protein migrates as 27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 1 mM TCEP, 0.5 M Arginine, pH 7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human FGF-10, His,Avitag (Cat. No. FG0-H81Q7) SDS-PAGE gel

Biotinylated Human FGF-10, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Biotinylated Human FGF-10, His,Avitag (Cat. No. FG0-H81Q7) immobilized on SA Chip can bind Human FGFR2 (IIIb), His Tag (Cat. No. FGB-H5223) with an affinity constant of 5.02 μM as determined in a SPR assay (Biacore 8K) (QC tested).

  • Background
    Fibroblast Growth Factor 10 (FGF 10) is an evolutionary conserved secreted growth factor mediating mostly mesenchymal to epithelial signaling. FGF 10 belongs to the FGF 7 subfamily and shares similar biochemical and amino acid sequences with its constituent members (FGF3, FGF 7 and FGF 22). As a paracrine FGF, FGF 10 elicits its biological responses by activating the fibroblast growth factor receptor 2b (FGF R 2b), is crucial for governing proximal distal outgrowth as well as patterning and acts upstream of the known apical ectodermal ridge (AER) marker FGF 8. FGF10 is also implicated in pancreatic cancer, and that overexpression of FGFR2b is associated with metastatic invasion.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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