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Product Details
Synonyms
CD71, TFRC, TFR, sTfR, TfR1, Trfr, T9, p90
Source
Biotinylated Cynomolgus Transferrin R, His Tag, primary amine labeling (TFR-C8249) is expressed from human 293 cells (HEK293). It contains AA Cys 89 - Phe 760 (Accession # G8F602-1). It is the biotinylated form of Cynomolgus Transferrin R, His Tag (Cat. No. TFR-C524a).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 77.1 kDa. The protein migrates as 70-90 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Cynomolgus Transferrin R, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Cynomolgus Transferrin R, His Tag, primary amine labeling (Cat. No. TFR-C8249) is more than 85% and the molecular weight of this protein is around 155-190 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Cynomolgus Transferrin R, His Tag, primary amine labeling (Cat. No. TFR-C8249) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse Transferrin, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. TRN-M525b) with a linear range of 0.3-10 ng/mL (QC tested).
Protocol
Immobilized Mouse Transferrin, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. TRN-M525b) at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus Transferrin R, His Tag, primary amine labeling (Cat. No. TFR-C8249) with a linear range of 0.3-10 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Biotinylated Cynomolgus Transferrin R, His Tag, primary amine labeling (Cat. No. TFR-C8249) captured on Biotin CAP-Series S Sensor Chip can bind Rhesus macaque Transferrin Protein, His Tag (Cat. No. TRN-R52H3) with an affinity constant of 2.51 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Transferrin receptor protein 1
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
15 Details
- Highest Development Stage：
Approved
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