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Your Position: Home > All Other Proteins > Thrombopoietin R > THR-H52H7

Human Thrombopoietin R / TPO R Protein, His Tag (MALS verified)

  • Synonym
    MPL,C-MPL,CD110,MPLV,THCYT2,TPOR
  • Source
    Human Thrombopoietin R, His Tag (THR-H52H7) is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Trp 491 (Accession # P40238-1).
    Predicted N-terminus: Gln 26
  • Molecular Characterization
    Online(Gln 26 - Trp 491) P40238-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 54.4 kDa. The protein migrates as 60-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Thrombopoietin R, His Tag (Cat. No. THR-H52H7) SDS-PAGE gel

Human Thrombopoietin R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human Thrombopoietin R, His Tag (Cat. No. ) MALS images

The purity of Human Thrombopoietin R, His Tag (Cat. No. THR-H52H7) is more than 95% and the molecular weight of this protein is around 60-74 kDa verified by SEC-MALS.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human Thrombopoietin R, His Tag (Cat. No. THR-H52H7) captured on CM5 Chip via anti-His antibody can bind Human Thrombopoietin, Tag Free (Cat. No. THN-H5214) with an affinity constant of 14.9 nM as determined in SPR assay (Biacore T200) (Routinely tested).

  • Background
    Thrombopoietin R, also known as TPO-R, is expressed predominantly on the surface of MKs, platelets, hemangioblasts, and hematopoietic stem cells (HSCs). Binding of TPO to the megakaryocyte TPO-R leads to different effects: prevention of megakaryocyte apoptosis; increased megakaryocyte number, size, and ploidy; increasing rate of megakaryocyte maturation; and internalization of the TPO/TPO-R complex. Thrombopoietin R involved in multiple signal transduction pathways, such as JAK, STAT, and MAP kinase.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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