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Your Position: Home > Recombinant Antibodies > Spike RBD > SPD-M110

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (AS110) (Delta Specific) (MALS verified)

  • Source
    The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with Spike RBD F(ab')2. The antibody is specific against the Delta (B.1.617.2/AY.4) variant of SARS-CoV-2, and has no binding with the spike RBD of the wild type virus and the Alpha (B.1.1.7), Beta (B.1.351), Gamma (P.1), Omicron (B.1.1.529/BA.1) variant.
  • Isotype
    Mouse IgG/kappa
  • Specificity
    This product is a specific antibody against SARS-CoV-2 Spike protein RBD domain. Cross-reactivity with Spike protein RBD domain of other coronaviruses, including SARS-CoV, MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1, has not been tested.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Delta Specific) (Cat. No. SPD-M110) SDS-PAGE gel

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Delta Specific) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Delta Specific) (Cat. No. ) MALS images

The purity of Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Delta Specific) (Cat. No. SPD-M110) is more than 90% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.

Bioactivity-ELISA
Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Delta Specific)Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Delta Specific) (Cat. No. SPD-M110) ELISA bioactivity

Immobilized SARS-CoV-2 Spike RBD (Delta, Cat. No. SPD-C52Hh) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Cat. No.SPD-M110) with a linear range of 0.1-3 ng/mL. The antibody does not bind Spike RBD of WT (Cat. No. SPD-C52H1), Alpha (Cat. No. SPD-C52Hn), Beta (Cat. No. SPD-C52Hp), Gamma (Cat. No. SPD-C52Hr) and Omicron (Cat. No. SPD-C522e) (QC tested).

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Delta Specific)Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Delta Specific) (Cat. No. SPD-M110) ELISA bioactivity

Immobilized SARS-CoV-2 Spike Trimer (Delta, Cat. No. SPN-C52He) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Cat. No. SPD-M110) with a linear range of 0.1-3 ng/mL. The antibody does not bind spike protein of WT (Cat. No. SPN-C52H7), Alpha (Cat. No. SPN-C52H6), Beta (Cat. No. SPN-C52Hk), Gamma (Cat. No. SPN-C52Hg) and Omicron (Cat. No. SPN-C52Hz) (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Delta Specific) (Cat. No. SPD-M110) captured on CM5 chip via Anti-mouse antibodies surface can bind SARS-CoV-2 Spike RBD (L452R, T478K), His Tag (Cat. No. SPD-C52Hh) with an affinity constant of 3.29 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Human_FcRn_Heterodimer_Protein_SPR

Anti-SARS-CoV-2 Spike RBD Antibody, Mouse IgG1 (Delta Specific) (Cat. No. SPD-M110) captured on CM5 chip via Anti-mouse antibodies surface can bind SARS-CoV-2 Spike Trimer, His Tag (Cat. No. SPN-C52He) with an affinity constant of 5.59 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 2 business days

Price(USD) : $790.00

Price(USD) : $4980.00

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