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Your Position: Home > Antibodies for diagnostic > Nucleocapsid protein > NUN-BM272

Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (AM223) (MALS verified)

Based on the high binding affinity between biotin-streptavidin systems, our best seller SP-11 Streptavidin Coated Plates are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Source
    Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (AM223) is a chimeric monoclonal antibody recombinantly expressed from HEK293 cells, which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody was obtained from a mouse immunized with recombinant SARS-CoV-2 Nucleocapsid Protein. This chimeric antibody is purified by Protein A affinity chromatography.
  • Isotype
    Human IgG1/kappa
  • Specificity
    This product can recognize SARS-CoV-2 nucleocapsid protein. No cross-reactivity is detected with nucleocapsid protein of other coronaviruses, including MERS-CoV, HCoV-229E, HCoV-NL63, HCoV-OC43 and HCoV-HKU1.
  • Application
    This antibody can be paired with other Anti-SARS-CoV-2 nucleocapsid antibodies to detect SARS-CoV-2 nucleocapsid protein in sandwich ELISA or LFA assay.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4.

    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. For long term storage, the product is stable for up to 3 years at -70°C from date of receipt;
    2. For short term storage, the product is stable for up to 12 months at 2-8°C from date of receipt.
  • Shipping
    This product is supplied and shipped as sterile liquid solution with blue ice, please inquire the shipping cost.
SDS-PAGE
Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (AM223) (Cat. No. NUN-BM272) SDS-PAGE gel

Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (AM223) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (AM223) (Cat. No. ) MALS images

The purity of Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody, Chimeric mAb, Human IgG1 (AM223) (Cat. No. NUN-BM272) is more than 95% and the molecular weight of this protein is around 135-160 kDa verified by SEC-MALS.

  • Background
    Nucleocapsid (N) protein is the most abundant protein found in coronavirus. CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways. It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M. E., et al. 2017, Virology; 517: 62-68). The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics. Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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