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This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 41.5 kDa. The protein migrates as 55-60kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mouse CD28, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Mouse CD28, Fc Tag (Cat. No. CD8-M5258) is more than 90% and the molecular weight of this protein is around 95-115 kDa verified by SEC-MALS.
Loaded Mouse CD28, Fc Tag (Cat. No. CD8-M5258) on Protein A Biosensor, can bind with Mouse B7-2, His Tag (Cat. No. CD6-M52H0) an affinity constant of 7.9 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).
Loaded Mouse CD28, Fc Tag (Cat. No. CD8-M5258) on Protein A Biosensor, can bind with Mouse B7-1, His Tag (Cat. No. CD0-M5228) an affinity constant of 8.1 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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