Biotinylated Rabbit FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of FCGRT and B2M, has a calculated MW of 33.8 kDa (FCGRT) & 11.7 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) and subunit Beta-2 microglobulin (B2M) contains no tag at the C-terminus. The reducing (R) protein migrates as 35-40 kDa (FCGRT) & 12 kDa (B2M) respectively due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Rabbit FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Rabbit FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free (Cat. No. FCM-R82W6) is more than 90% and the molecular weight of this protein is around 42-62 kDa verified by SEC-MALS.
Biotinylated Rabbit FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free (Cat. No. FCM-R82W6) captured on Biotin CAP-Series S Sensor Chip can bind Herceptin® with an affinity constant of 1.05 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Immobilized Biotinylated Rabbit FCGRT&B2M Heterodimer Protein, His,Avitag&Tag Free (Cat. No. FCM-R82W6) on SA Chip can bind Herceptin® with an affinity constant of 1.53 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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