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Your Position: Home > Cytokines-old > CCL1 > CC1-H5254

Human CCL1 / I-309 Protein, Fc Tag (MALS verified)

  • Synonym
    CCL1/I-309/C-C motif chemokine 1
  • Source
    Human CCL1, Fc Tag (CC1-H5254) is expressed from human 293 cells (HEK293). It contains AA Lys 24 - Lys 96 (Accession # P22362-1).
    Predicted N-terminus: Lys 24
  • Molecular Characterization
    Online(Lys 24 - Lys 96) P22362-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 34.9 kDa. The protein migrates as 35-44 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human CCL1, Fc Tag (Cat. No. CC1-H5254) SDS-PAGE gel

Human CCL1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human CCL1, Fc Tag (Cat. No. ) MALS images

The purity of Human CCL1, Fc Tag (Cat. No. CC1-H5254) is more than 95% and the molecular weight of this protein is around 68-88 kDa verified by SEC-MALS.

  • Background
    Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL1, a member of the CC subfamily, is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine (C-C motif) receptor 8.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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