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Product Details
Synonyms
IL3, MCGF, MGC79398, MGC79399, MULTI-CSF, Interleukin-3
Source
Mouse IL-3 Protein, His Tag (IL3-M52H8) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Cys 166 (Accession # P01586-1).
Predicted N-terminus: Ala 27
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 17.6 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Mouse IL-3 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Mouse IL-3 Protein, His Tag (Cat. No. IL3-M52H8) is more than 85% and the molecular weight of this protein is around 20-32 kDa verified by SEC-MALS.
Report
Bioactivity-SPR
Immobilized Mouse IL-3 R alpha, His Tag (Cat. No. ILA-M52H4) on CM5 Chip can bind Mouse IL-3 Protein, His Tag (Cat. No. IL3-M52H8) with an affinity constant of 0.929 μM as determined in a SPR assay (Biacore T200) (QC tested).
Protocol
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