This protein carries a polyhistidine tag at the C-terminus
The protein has a calculated MW of 74.9 kDa. The protein migrates as 85-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human VCAM-1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human VCAM-1, His Tag (Cat. No. VC1-H5224) is more than 90% and the molecular weight of this protein is around 78-106 kDa verified by SEC-MALS.
Immobilized Human VCAM-1, His Tag (Cat. No. VC1-H5224) at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGA4&ITGB1 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT1-H82W1) with a linear range of 4-78 ng/mL (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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