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Your Position: Home > TME Relevant Pathway > HGF R > MET-M5254

Mouse HGF R / c-MET Protein, Fc Tag (MALS verified)

  • Synonym
    MET,AUTS9,HGFR,RCCP2,c-Met
  • Source
    Mouse HGF R, Fc Tag (MET-M5254) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Ala 931 (Accession # P16056-1).
    Predicted N-terminus: Glu 25
  • Molecular Characterization
    Online(Glu 25 - Ala 931) P16056-1

    Mouse HGF R, Fc Tag is fused with Fc fragment of human IgG1 at the C-terminus. The mature form of HGF R is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 32.1 kDa (α chain) and 95.1 kDa (β chain Fc chimera). The protein migrates as 40-45 kDa (α chain) and 125-135 kDa (β chain Fc chimera) under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse HGF R, Fc Tag (Cat. No. MET-M5254) SDS-PAGE gel

Mouse HGF R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Mouse HGF R, Fc Tag (Cat. No. ) MALS images

The purity of Mouse HGF R, Fc Tag (Cat. No. MET-M5254) is more than 90% and the molecular weight of this protein is around 254-311 kDa verified by SEC-MALS.

Bioactivity-ELISA
Mouse HGF R, Fc TagMouse HGF R, Fc Tag (Cat. No. MET-M5254) ELISA bioactivity

Immobilized Human HGF, His Tag (Cat. No. HGF-H52H3) at 2 μg/mL (100 μL/well) can bind Mouse HGF R, Fc Tag (Cat. No. MET-M5254) with a linear range of 0.2-10 ng/mL (QC tested).

  • Background
    Hepatocyte growth factor receptor (HGFR) is also known as mesenchymal-epithelial transition factor (MET), c-Met, and is a glycosylated receptor tyrosine kinase that plays a central role in epithelial morphogenesis and cancer development. HGFR protein possesses tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. HGFR is normally expressed by cells of epithelial origin, while expression of HGF is restricted to cells of mesenchymal origin. Upon HGF stimulation, HGFR induces several biological responses that collectively give rise to a program known as invasive growth. Abnormal HGFR activation in cancer correlates with poor prognosis, where aberrantly active HGFR triggers tumor growth, formation of new blood vessels (angiogenesis) that supply the tumor with nutrients, and cancer spread to other organs (metastasis). HGFR is deregulated in many types of human malignancies, including cancers of kidney, liver, stomach, breast, and brain. Normally, only stem cells and progenitor cells express HGFR, However, cancer stem cells are thought to hijack the ability of normal stem cells to express HGFR, and thus become the cause of cancer persistence and spread to other sites in the body. Various mutations in the HGFR gene are associated with papillary renal carcinoma. HGFR mediates a complex program known as invasive growth. Activation of HGFR triggers mitogenesis, and morphogenesis.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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