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This protein carries a human IgG1 Fc tag at the N-terminus
The protein has a calculated MW of 111.8 kDa. The protein migrates as 120-135 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Semaphorin 3A, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human Semaphorin 3A, Fc Tag (Cat. No. SEA-H5259) is more than 90% and the molecular weight of this protein is around 220-269 kDa verified by SEC-MALS.
Immobilized Human Neuropilin-1, His Tag (Cat. No. NR1-H5228) at 5 μg/mL (100 μL/well) can bind Human Semaphorin 3A, Fc Tag (Cat. No. SEA-H5259) with a linear range of 2-78 ng/mL (QC tested).
Immobilized Biotinylated Human Neuropilin-1, His,Avitag (Cat. No. NR1-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Semaphorin 3A, Fc Tag (Cat. No. SEA-H5259) with a linear range of 0.6-20 ng/mL (QC tested).
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