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Your Position: Home > All Other Proteins > Semaphorin 3A > SEA-H5259

Human Semaphorin 3A / SEMA3A Protein, Fc Tag (MALS verified)

  • Synonym
    Semaphorin 3A,(semaphorin) 3A,coll-1,collapsin 1,Hsema-I,Hsema-III,MGC133243,sema domain,immunoglobulin domain (Ig),short basic domain,secreted,Sema III,SEMA1, Sema3A,SEMAD,SEMAIII,SEMAL,Semaphorin 3A,semaphorin D,Semaphorin III,semaphorin L,semaphorin-3A
  • Source
    Human Semaphorin 3A, Fc Tag (SEA-H5259) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Val 771 (Accession # Q14563-1(R551A, R555A, R730A, K731A, R733A, R734A, R757A, R760A)).
    Predicted N-terminus: Pro
  • Molecular Characterization

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 111.8 kDa. The protein migrates as 120-135 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Semaphorin 3A, Fc Tag (Cat. No. SEA-H5259) SDS-PAGE gel

Human Semaphorin 3A, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human Semaphorin 3A, Fc Tag (Cat. No. ) MALS images

The purity of Human Semaphorin 3A, Fc Tag (Cat. No. SEA-H5259) is more than 90% and the molecular weight of this protein is around 220-269 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human Semaphorin 3A, Fc TagHuman Semaphorin 3A, Fc Tag (Cat. No. SEA-H5259) ELISA bioactivity

Immobilized Human Neuropilin-1, His Tag (Cat. No. NR1-H5228) at 5 μg/mL (100 μL/well) can bind Human Semaphorin 3A, Fc Tag (Cat. No. SEA-H5259) with a linear range of 2-78 ng/mL (QC tested).

Human Semaphorin 3A, Fc TagHuman Semaphorin 3A, Fc Tag (Cat. No. SEA-H5259) ELISA bioactivity

Immobilized Biotinylated Human Neuropilin-1, His,Avitag (Cat. No. NR1-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Semaphorin 3A, Fc Tag (Cat. No. SEA-H5259) with a linear range of 0.6-20 ng/mL (QC tested).

  • Background
    Semaphorin-3A (SEMA3A) is also known as Semaphorin III, which belongs to the semaphorin family. SEMA3A contains 1 Ig-like C2-type (immunoglobulin-like) domain, one PSI domain amd one sema domain. Semaphorin-3A is a secreted proteins, or chemorepulser, secreted by surrounding tissues to guide migrating cells and axons in the developing nervous system of an organism which is critical for the precise formation of neurons and vasculature. SEMA3A can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of SEMA3A is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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