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Your Position: Home > Cytokines > IL-17F > ILF-H82Q9

Biotinylated Human IL-17F Protein, His,Avitag™ (MALS verified)

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    IL-17F,Interleukin-17F,Cytokine ML-1
  • Source
    Biotinylated Human IL-17F, His,Avitag (ILF-H82Q9) is expressed from human 293 cells (HEK293). It contains AA Arg 31 - Gln 163 (Accession # Q96PD4-1).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Arg 31 - Gln 163) Q96PD4-1

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 18.5 kDa. The protein migrates as 25-28 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human IL-17F, His,Avitag (Cat. No. ILF-H82Q9) SDS-PAGE gel

Biotinylated Human IL-17F, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human IL-17F, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human IL-17F, His,Avitag (Cat. No. ILF-H82Q9) is more than 90% and the molecular weight of this protein is around 37-55 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human IL-17F, His,AvitagBiotinylated Human IL-17F, His,Avitag (Cat. No. ILF-H82Q9) ELISA bioactivity

Immobilized Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-17F, His,Avitag (Cat. No. ILF-H82Q9) with a linear range of 0.6-78 ng/mL (QC tested).

Biotinylated Human IL-17F, His,AvitagBiotinylated Human IL-17F, His,Avitag (Cat. No. ILF-H82Q9) ELISA bioactivity

Immobilized Biotinylated Human IL-17F, His,Avitag (Cat. No. ILF-H82Q9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116)precoated (0.5 μg/well) plate can bind Human IL-17 RA, Fc Tag (MALS verified) (Cat. No. ILA-H5257) with a linear range of 1-78 ng/mL (Routinely tested).

Biotinylated Human IL-17F, His,AvitagBiotinylated Human IL-17F, His,Avitag (Cat. No. ILF-H82Q9) ELISA bioactivity

Immobilized Biotinylated Human IL-17F, His,Avitag (Cat. No. ILF-H82Q9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with a linear range of 2-156 ng/mL (Routinely tested).

  • Background
    Interleukin-17F (IL17F) is also known as Interleukin-24 (IL24), Cytokine ML-1, is a secreted disulfide-linked homodimer which belongs to the IL-17 family. There are at least six members of the IL-17 family in humans and in mice: IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25) and IL-17F (also called IL-24). IL-17 represents a family of structurally related cytokines that share a highly conserved C-terminal region but differ from one another in their N-terminal regions and in their distinct biological roles. IL-17F / IL-24 is expressed in activated, but not resting, CD4+ T-cells and activated monocytes. IL17F / Interleukin-24 stimulates the production of other cytokines such as IL-6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. IL17F / IL-24 stimulates PBMC and T-cell proliferation and Inhibits angiogenesis. Defects in IL17F are the cause of familial candidiasis type 6 (CANDF6).
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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