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Your Position: Home > All Other Proteins > C-Reactive Protein > CRP-H82E4

Biotinylated Human C-Reactive Protein (CRP), His,Avitag™ (MALS verified)

Based on the high binding affinity between biotin-streptavidin systems, our best seller SP-11 Streptavidin Coated Plates are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    CRP,C-reactive Protein
  • Source
    Biotinylated Human C-Reactive Protein, His,Avitag (CRP-H82E4) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Pro 224 (Accession # P02741-1).
    Predicted N-terminus: Gln 19
  • Molecular Characterization
    Online(Gln 19 - Pro 224) P02741-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 26.6 kDa. The protein migrates as 29 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 1 M Nacl, pH8.0. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human C-Reactive Protein, His,Avitag (Cat. No. CRP-H82E4) SDS-PAGE gel

Biotinylated Human C-Reactive Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human C-Reactive Protein, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human C-Reactive Protein, His,Avitag (Cat. No. CRP-H82E4) is more than 90% and the molecular weight of this protein is around 140-155 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human C-Reactive Protein, His,AvitagBiotinylated Human C-Reactive Protein, His,Avitag (Cat. No. CRP-H82E4) ELISA bioactivity

Immobilized Human CD32a (H167), His Tag (Cat. No. CD1-H5223) at 5 μg/mL (100 μL/well) can bind Biotinylated Human C-Reactive Protein, His,Avitag (Cat. No. CRP-H82E4) with a linear range of 0.02-1.25 μg/mL (QC tested).

  • Background
    C-reactive protein (CRP) is a member of the pentraxin family of proteins that are characterized by a cyclic pentameric structure. Human CRP gene encodes a 224 amino acids precursor. The mature human CRP protein has 206 amino acids that are noncovalently linked to form the pentameter. Human CRP shares 71% and 64% amino acid sequence homology with mouse and rat respectively. CRP, synthesized by hepatocytes, is a major acute phase serum protein in human. IL6, IL1 and glucocorticoids are the major inducer of the CRP gene. The physiological role of CRP is to bind to phosphocholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system. CRP binds to phosphocholine on microbes and damaged cells and enhances phagocytosis by macrophages. Thus, CRP participates in the clearance of necrotic and apoptotic cells. CRP rises up to 50,000-fold in acute inflammation, such as infection. It rises above normal limits within 6 hours, and peaks at 48 hours. Its half-life is constant, and therefore its level is mainly determined by the rate of production. It has been shown that high levels of CRP in humans is associated with an increased risk of cardiovascular diseases.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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