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Product Details
Synonyms
TNFRSF1B, CD120b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR80, p75, p75TNFR
Source
Cynomolgus / Rhesus macaque TNFR2, His Tag (TN2-C52H7) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Asp 257 (Accession # XP_005544817.1). In the region Leu 23 - Asp 257, the amino acid sequences of Cynomolgus and Rhesus macaque TNFR2 are identical.
Predicted N-terminus: Leu 23
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.9 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Cynomolgus / Rhesus macaque TNFR2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling (Cat. No. TNA-H8211) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Cynomolgus / Rhesus macaque TNFR2, His Tag (Cat. No. TN2-C52H7) with a linear range of 0.5-8 ng/mL (QC tested).
Protocol
Immobilized Cynomolgus / Rhesus macaque TNFR2, His Tag (Cat. No. TN2-C52H7) at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified) (Cat. No. TNA-H82E3) with a linear range of 0.2-10 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor receptor superfamily member 1B
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
8 Details
- Highest Development Stage：
Approved
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