This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 99.2 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human ROBO1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human ROBO1, His,Avitag (Cat. No. RB1-H82E5) is more than 90% and the molecular weight of this protein is around 100-122 kDa verified by SEC-MALS.
Immobilized Anti-ROBO1 Antibody, Mouse IgG1 at 1 μg/mL (100 μL/well) can bind Biotinylated Human ROBO1, His,Avitag (Cat. No. RB1-H82E5) with a linear range of 2-31 ng/mL (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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