This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 26.7 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (Cat. No. SPD-C522g) is more than 90% and the molecular weight of this protein is around 32-48 kDa verified by SEC-MALS.
Immobilized SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (Cat. No. SPD-C522g) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122)) with a linear range of 0.8-13 ng/mL (QC tested).
Serial dilutions of Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM122) (Trehalose free) (Cat. No. S1N-M12A1) can inhibit SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (MALS verified) (Cat. No. SPD-C522g) from binding Human ACE2. The half maximal inhibitory concentration (IC50) is 1.844 μg/mL (Routinely tested).
Serial dilutions of Anti-SARS-CoV-2 Spike RBD Broadly Neutralizing Antibody, Human IgG1 (MALS verified) (Cat. No. SPD-M265) can inhibit SARS-CoV-2 Spike RBD, His Tag (BA.2/Omicron) (MALS verified) (Cat. No. SPD-C522) from binding Human ACE2. The half maximal inhibitory concentration (IC50) is 2.038 μg/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $400.00
Price(USD) : $2600.00
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