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Your Position: Home > All Other Proteins > Flt-3 > FL3-HF255

FITC-Labeled Human Flt-3 / Flk-2 Protein, Fc Tag

  • Synonym
    Flt-3,Flk-2,STK-1,CD135,FLK2,FLT-3
  • Source
    FITC-Labeled Human Flt-3, Fc Tag (FL3-HF255) is expressed from human 293 cells (HEK293). It contains AA Asn 27 - Ser 543 (Accession # P36888-1).
    Predicted N-terminus: Asn 27
  • Molecular Characterization
    Online(Asn 27 - Ser 543) P36888-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 84.8 kDa. The protein migrates as 110-130 kDa and 250 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Conjugate

    FITC

    Excitation source: 488 nm spectral line, argon-ion laser

    Excitation Wavelength: 488 nm

    Emission Wavelength: 535 nm

  • Labeling
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
  • FITC:Protein Ratio
    The FITC to protein molar ratio is 2-4.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FITC-Labeled Human Flt-3, Fc Tag (Cat. No. FL3-HF255) SDS-PAGE gel

FITC-Labeled Human Flt-3, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
FITC-Labeled Human Flt-3, Fc TagFITC-Labeled Human Flt-3, Fc Tag (Cat. No. FL3-HF255) ELISA bioactivity

Immobilized ActiveMax® Human Flt-3 Ligand, Tag Free (Cat. No. FLL-H5218) at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human Flt-3, Fc Tag (Cat. No. FL3-HF255) with a linear range of 0.625-20 μg/mL (QC tested).

FITC-Labeled Human Flt-3, Fc TagFITC-Labeled Human Flt-3, Fc Tag (Cat. No. FL3-HF255) ELISA bioactivity

Immobilized ActiveMax® Human Flt-3 Ligand, Tag Free (Cat. No. FLL-H5218) at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human Flt-3, Fc Tag (Cat. No. FL3-HF255) with a linear range of 0.2-13 ng/mL (Routinely tested).

  • Background
    Flt-3(Receptor-type tyrosine-protein kinase FLT3) is also known as FLK-2(Fetal liver kinase-2), STK-1(Stem cell tyrosine kinase 1), CD135. FLT3 is a cytokine receptor which belongs to the receptor tyrosine kinase class III. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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