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Your Position: Home > Receptor > Transferrin R > TFR-M8249

Biotinylated Mouse Transferrin R / CD71 Protein, His Tag, ultra sensitivity (primary amine labeling) (MALS verified)

Recommended Hot Products: SP-11 Streptavidin Coated Plates, Clear, 96-Well  STN-N5116 Recombinant Streptavidin  SMB-B01 Magnetic Beads™ Streptavidin 
Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    CD71,TFRC,TFR,sTfR,TfR1,Trfr,T9,p90
  • Source
    Biotinylated Mouse Transferrin R, His Tag, primary amine labeling (TFR-M8249) is expressed from human 293 cells (HEK293). It contains AA Cys 89 - Phe 763 (Accession # Q62351-1). It is the biotinylated form of Mouse Transferrin R, His Tag(Cat. No. TFR-M524b)
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Cys 89 - Phe 763) Q62351-1

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 77.8 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse Transferrin R, His Tag, primary amine labeling (Cat. No. TFR-M8249) SDS-PAGE gel

Biotinylated Mouse Transferrin R, His Tag, primary amine labeling on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Mouse Transferrin R, His Tag, primary amine labeling (Cat. No. ) MALS images

The purity of Biotinylated Mouse Transferrin R, His Tag, primary amine labeling (Cat. No. TFR-M8249) is more than 95% and the molecular weight of this protein is around 145-177 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Mouse Transferrin R, His Tag, primary amine labelingBiotinylated Mouse Transferrin R, His Tag, primary amine labeling (Cat. No. TFR-M8249) ELISA bioactivity

Immobilized Biotinylated Mouse Transferrin R, His Tag, primary amine labeling (Cat. No. TFR-M8249) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse Transferrin, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. TRN-M525b) with a linear range of 0.2-16 ng/mL (QC tested).

Biotinylated Mouse Transferrin R, His Tag, primary amine labelingBiotinylated Mouse Transferrin R, His Tag, primary amine labeling (Cat. No. TFR-M8249) ELISA bioactivity

Immobilized Mouse Transferrin, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. TRN-M525b) at 2 μg/mL (100 μL/well) can bind Biotinylated Mouse Transferrin R, His Tag, primary amine labeling (Cat. No. TFR-M8249) with a linear range of 0.5-63 ng/mL (Routinely tested).

  • Background
    CD antigen CD71 is also known as Transferrin receptor protein 1, TfR, sTfR, p90, TfR1, Trfr, which belongs to the peptidase M28 family and M28B subfamily. CD71 /TFR contains one PA (protease associated) domain. CD71 / TfR1 is required for iron delivery from transferrin to cells. CD71 is a potential new target in cases of human leukomia & lymphoma. CD71 /TFRC / TfR has been shown to interact with GABARAP and HFE.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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