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Your Position: Home > TME Relevant Pathway > FGF R3 (IIIc) > FGC-H5256

Human FGF R3 (IIIc) / FGF R3C / CD333 Protein, Fc Tag (MALS verified)

  • Synonym
    FGF R3 (IIIc),FGF R3C,CD333
  • Source
    Human FGF R3 (IIIc), Fc Tag (FGC-H5256) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 375 (Accession # P22607-1).
    Predicted N-terminus: Glu 23
  • Molecular Characterization
    Online(Glu 23 - Gly 375) P22607-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 64.6 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human FGF R3 (IIIc), Fc Tag (Cat. No. FGC-H5256) SDS-PAGE gel

Human FGF R3 (IIIc), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human FGF R3 (IIIc), Fc Tag (Cat. No. ) MALS images

The purity of Human FGF R3 (IIIc), Fc Tag (Cat. No. FGC-H5256) is more than 90% and the molecular weight of this protein is around 148-180 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human FGF R3 (IIIc), Fc TagHuman FGF R3 (IIIc), Fc Tag (Cat. No. FGC-H5256) ELISA bioactivity

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 5 μg/mL (100 μL/well) can bind Human FGF R3 (IIIc), Fc Tag (Cat. No. FGC-H5256) with a linear range of 1-39 ng/mL (QC tested).

Human FGF R3 (IIIc), Fc TagHuman FGF R3 (IIIc), Fc Tag (Cat. No. FGC-H5256) ELISA bioactivity

Immobilized Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human FGF R3 (IIIc), Fc Tag (Cat. No. FGC-H5256) with a linear range of 2-39 ng/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human FGF R3 (IIIc), Fc Tag immobilized (Cat. No. FGC-H5256) on CM5 Chip can bind Human FGF acidic, Tag Free (Cat. No. AFF-H4116) with an affinity constant of 0.44 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

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