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Your Position: Home > TME Relevant Pathway > FGF R3 (IIIb) > FGB-H5259

Human FGF R3 (IIIb) / FGF R3B / CD333 Protein, Fc Tag (MALS verified)

  • Synonym
    FGF R3 (IIIb),FGF R3B,CD333
  • Source
    Human FGF R3 (IIIb), Fc Tag (FGB-H5259) is expressed from human 293 cells (HEK293). It contains AA Glu 23 - Gly 377 (Accession # P22607-2).
    Predicted N-terminus: Glu 23
  • Molecular Characterization
    Online(Glu 23 - Gly 377) P22607-2

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 65.0 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human FGF R3 (IIIb), Fc Tag (Cat. No. FGB-H5259) SDS-PAGE gel

Human FGF R3 (IIIb), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human FGF R3 (IIIb), Fc Tag (Cat. No. ) MALS images

The purity of Human FGF R3 (IIIb), Fc Tag (Cat. No. FGB-H5259) is more than 90% and the molecular weight of this protein is around 132-162 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human FGF R3 (IIIb), Fc TagHuman FGF R3 (IIIb), Fc Tag (Cat. No. FGB-H5259) ELISA bioactivity

Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 5 μg/mL (100 μL/well) can bind Human FGF R3 (IIIb), Fc Tag (Cat. No. FGB-H5259) with a linear range of 2-39 ng/mL (QC tested).

Human FGF R3 (IIIb), Fc TagHuman FGF R3 (IIIb), Fc Tag (Cat. No. FGB-H5259) ELISA bioactivity

Immobilized Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human FGF R3 (IIIb), Fc Tag (Cat. No. FGB-H5259) with a linear range of 1-39 ng/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human FGF R3 (IIIb), Fc Tag (Cat. No. FGB-H5259) immobilized on CM5 Chip can bind Human FGF acidic, Tag Free (Cat. No. AFF-H4116) with an affinity constant of 5.91 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

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