This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 65.0 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human FGF R3 (IIIb), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human FGF R3 (IIIb), Fc Tag (Cat. No. FGB-H5259) is more than 90% and the molecular weight of this protein is around 132-162 kDa verified by SEC-MALS.
Immobilized Human FGF basic, Tag Free (Cat. No. BFF-H4117) at 5 μg/mL (100 μL/well) can bind Human FGF R3 (IIIb), Fc Tag (Cat. No. FGB-H5259) with a linear range of 2-39 ng/mL (QC tested).
Human FGF R3 (IIIb), Fc Tag (Cat. No. FGB-H5259) immobilized on CM5 Chip can bind Human FGF acidic, Tag Free (Cat. No. AFF-H4116) with an affinity constant of 5.91 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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