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Your Position: Home > FcR > Fc gamma RIIA / CD32a > CDA-H82E5

Biotinylated Human Fc gamma RIIA / CD32a (R167) Protein, His,Avitag™ (MALS & SPR verified)

Recommended Hot Products: SP-11 Streptavidin Coated Plates, Clear, 96-Well  STN-N5116 Recombinant Streptavidin  SMB-B01 Magnetic Beads™ Streptavidin 
Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    CD32a,FCGR2A,CD32,FCG2 ,FCGR2A1,IGFR2
  • Source
    Biotinylated Human CD32a (H167R), His,Avitag (CDA-H82E5) is expressed from human 293 cells (HEK293). It contains AA Ala 36 - Ile 218 (Accession # NP_001129691.1).
    Predicted N-terminus: Ala 36
  • Molecular Characterization
    Online(Ala 36 - Ile 218) NP_001129691.1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 23.9 kDa. The protein migrates as 30-34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD32a (R167), His,Avitag™ (Cat. No. CDA-H82E5) SDS-PAGE gel

Biotinylated Human CD32a (R167), His,Avitag™ on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human CD32a (R167), His,Avitag™ (Cat. No. ) MALS images

The purity of Biotinylated Human CD32a (R167), His,Avitag™ (Cat. No. CDA-H82E5) is more than 90% and the molecular weight of this protein is around 23-35 kDa verified by SEC-MALS.

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Biotinylated Human CD32a (H167R), His,Avitag (Cat. No. CDA-H82E5) captured on Biotin CAP-Series S Sensor Chip can bind Rituximab with an affinity constant of 2.53 μM as determined in a SPR assay (Biacore 8K) (QC tested).

Human_FcRn_Heterodimer_Protein_SPR

Biotinylated Human CD32a (H167R), His,Avitag (Cat. No. CDA-H82E5) immobilized on SA Chip can bind Rituximab with an affinity constant of 0.802 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

  • Background
    Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets and dendritic cells. CD32A is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells), where inhibitory ITIM­bearing receptors may also be coexpressed and co­engaged by specific ligands. CD32A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as CD32B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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