This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 63.9 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human IL-18 R1, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human IL-18 R1, Fc,Avitag (Cat. No. IL1-H82F9) is more than 85% and the molecular weight of this protein is around 145-175 kDa verified by SEC-MALS.
Human IL-18, Tag Free (Cat. No. IL8-H5113) immobilized on CM5 Chip can bind Biotinylated Human IL-18 R1, Fc,Avitag (Cat. No. IL1-H82F9) with an affinity constant of 0.628 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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