Login Register
icon_bulk_orderBulk order Acrobiosystems for English
0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Mutants > Spike protein > SPN-C5225
  • Synonym
    Spike,S protein,Spike glycoprotein,S glycoprotein
  • Source
    SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (SPN-C5225) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (A67V,H69del,V70del,T95I,G142D,V143del,Y144del,Y145del,N211del,L212I,G339D,S371F,S373P,S375F,D405N,K417N,N440K,G446S,S477N,T478K,E484A,Q493R,Q498R,N501Y,Y505H,D614G,H655Y,N679K,P681H,N764K,D796Y,Q954H,N969K,R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.3; GISAID clade: GRA; Nextstrain clade: 21M). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
    Predicted N-terminus: Val 16
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 137.7 kDa. The protein migrates as 160-200 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (Cat. No. SPN-C5225) SDS-PAGE gel

SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (Cat. No. ) MALS images

The purity of SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (Cat. No. SPN-C5225) is more than 90% and the molecular weight of this protein is around 485-535 kDa verified by SEC-MALS.

Bioactivity-ELISA
SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron)SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (Cat. No. SPN-C5225) ELISA bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (Cat. No. SPN-C5225) with a linear range of 0.078-2.5 μg/mL (QC tested).

SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron)SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (Cat. No. SPN-C5225) ELISA bioactivity

Immobilized SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (Cat. No. SPN-C5225) at 1 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.2-25 ng/mL (Routinely tested).

SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron)SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (Cat. No. SPN-C5225) ELISA bioactivity

Immobilized SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (Cat. No. SPN-C5225) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122)) with a linear range of 0.1-13 ng/mL (Routinely tested).

SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron)SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (Cat. No. SPN-C5225) ELISA bioactivity

Immobilized SARS-CoV-2 Spike Trimer, His Tag (BA.3/Omicron) (Cat. No. SPN-C5225) at 1 μg/mL (100 μL/well) can bind Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Mouse IgG1 (AM122) (Cat. No. SCT-M369)) with a linear range of 0.1-6 ng/mL (Routinely tested).

  • Background
    It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)


ETD of in-stock products: 2 business days

Price(USD) : $450.00

Price(USD) : $3080.00

Promotion & Exhibitions



Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:3 Details
  • Latest Research Phase:Approved

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Related Products

New Product Launch

Questions & Comments

This web search service is supported by Google Inc.

totop
Call us
Call us
North America: +1 800-810-0816 (Toll Free)
Asia & Pacific: +86 400-682-2521
Fax
Fax
+1 888-377-6111
Address
Address
1 Innovation Way, Newark, DE 19711, USA

Leave a message