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Product Details
Synonyms
RANKL, CD254, TRANCE, OPGL, ODF
Source
Biotinylated Rat TNFSF11, His,Avitag (RAL-R82Q9) is expressed from human 293 cells (HEK293). It contains AA Leu 69 - Asp 318 (Accession # Q9ESE2-1).
Predicted N-terminus: His
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 31.8 kDa. The protein migrates as 35-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Rat TNFSF11, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Bioactivity-ELISA
Immobilized Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) at 1 μg/mL (100 μL/well) can bind Biotinylated Rat TNFSF11, His,Avitag (Cat. No. RAL-R82Q9) with a linear range of 0.5-16 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Rat TNFSF11, His,Avitag (Cat. No. RAL-R82Q9) at 2 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (1 μg/well) plate can bind Human Osteoprotegerin, Fc Tag (Cat. No. TNB-H5259) with a linear range of 0.1-16 ng/mL (Routinely tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Tumor necrosis factor ligand superfamily member 11
- Category：
- Approved Drugs：
18 Details
- Drugs in Clinical Trials：
18 Details
- Highest Development Stage：
Approved
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