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Your Position: Home > CKP > TNFSF11 > RAL-R82Q9

Biotinylated Rat TNFSF11 / RANKL / CD254 Protein, His,Avitag™

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Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    RANKL,CD254,TRANCE,OPGL,ODF
  • Source
    Biotinylated Rat TNFSF11, His,Avitag (RAL-R82Q9) is expressed from human 293 cells (HEK293). It contains AA Leu 69 - Asp 318 (Accession # Q9ESE2-1).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Leu 69 - Asp 318) Q9ESE2-1

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 31.8 kDa. The protein migrates as 35-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Rat TNFSF11, His,Avitag (Cat. No. RAL-R82Q9) SDS-PAGE gel

Biotinylated Rat TNFSF11, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Rat TNFSF11, His,AvitagBiotinylated Rat TNFSF11, His,Avitag (Cat. No. RAL-R82Q9) ELISA bioactivity

Immobilized Human RANK, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. RAK-H5251) at 1 μg/mL (100 μL/well) can bind Biotinylated Rat TNFSF11, His,Avitag (Cat. No. RAL-R82Q9) with a linear range of 0.5-16 ng/mL (QC tested).

Biotinylated Rat TNFSF11, His,AvitagBiotinylated Rat TNFSF11, His,Avitag (Cat. No. RAL-R82Q9) ELISA bioactivity

Immobilized Biotinylated Rat TNFSF11, His,Avitag (Cat. No. RAL-R82Q9) at 2 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (1 μg/well) plate can bind Human Osteoprotegerin, Fc Tag (Cat. No. TNB-H5259) with a linear range of 0.1-16 ng/mL (Routinely tested).

  • Background
    Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), TNF-related activation-induced cytokine (TRANCE), osteoprotegerin ligand (OPGL), and osteoclast differentiation factor (ODF), is known as a type II membrane protein and is a member of the tumor necrosis factor (TNF) superfamily. RANKL, through its ability to stimulate osteoclast formation and activity, is a critical mediator of bone resorption and overall bone density. Some findings also suggestion some cancer cells, particularly prostate cancer cells, can activate an increase in bone remodeling and ultimately increase overall bone production.[17] This increase in bone remodeling and bone production increases the overall growth of bone metastasizes. The overall control of bone remodeling is regulated by the binding of RANKL with its receptor or its decoy receptor, respectively, RANK and OPG.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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